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Molecular Cloning Forum DNA cloning forum. Discuss the cloning of plasmids, vectors, DNA, RNA, cDNA, proteins, and other molecules for expression, sub-cloning, and other methods. Also discuss digestion, ligation, transformation, plasmid preparation, cDNA library screening, and competent cells here.


Cloning Problems with Restriction Enzymes

Cloning Problems with Restriction Enzymes - Molecular Cloning Forum

Cloning Problems with Restriction Enzymes - DNA cloning forum. Discuss the cloning of plasmids, vectors, DNA, RNA, cDNA, proteins, and other molecules for expression, sub-cloning, and other methods. Also discuss digestion, ligation, transformation, plasmid preparation, cDNA library screening, and competent cells here.


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Old 01-03-2009, 11:56 PM
Pipette Filler
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Default Cloning Problems with Restriction Enzymes



Hey all,

How to reduce the star activity caused by BamHI. I am using two enzymes, BamHI and SphI.......however, I used NEB4 rather than the specified NEB3 specified by the company. After Digestion to confirm the presence of my insert, with these two enzymes its not reflecting my insert. I am assuming its star activity...Can anybdy tell me how to reduce this activity now in my vector with insert.
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