Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum

Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum (http://www.molecularstation.com/forum/)
-   Molecular Cloning Forum (http://www.molecularstation.com/forum/molecular-cloning-forum/)
-   -   Cloning Problems with Restriction Enzymes (http://www.molecularstation.com/forum/molecular-cloning-forum/68307-cloning-problems-restriction-enzymes.html)

manjula 01-03-2009 11:56 PM

Cloning Problems with Restriction Enzymes
 
Hey all,

How to reduce the star activity caused by BamHI. I am using two enzymes, BamHI and SphI.......however, I used NEB4 rather than the specified NEB3 specified by the company. After Digestion to confirm the presence of my insert, with these two enzymes its not reflecting my insert. I am assuming its star activity...Can anybdy tell me how to reduce this activity now in my vector with insert.


All times are GMT. The time now is 03:55 PM.

Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved

Page generated in 0.08811 seconds with 11 queries