Cloning Problems with Restriction Enzymes
How to reduce the star activity caused by BamHI. I am using two enzymes, BamHI and SphI.......however, I used NEB4 rather than the specified NEB3 specified by the company. After Digestion to confirm the presence of my insert, with these two enzymes its not reflecting my insert. I am assuming its star activity...Can anybdy tell me how to reduce this activity now in my vector with insert.
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