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| Molecular Cloning Forum DNA cloning forum. Discuss the cloning of plasmids, vectors, DNA, RNA, cDNA, proteins, and other molecules for expression, sub-cloning, and other methods. Also discuss digestion, ligation, transformation, plasmid preparation, cDNA library screening, and competent cells here. |
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| Does anyone know if E.coli can recognize mammalian signal peptides?. I cloned a mammalian protein with its signal peptide but I donīt see any expression in E. coli. Could it be possible it is been secreted to the media?. Thanks for your time. |
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| peptide , signal |
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