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signal peptide

signal peptide - Molecular Cloning Forum

signal peptide - DNA cloning forum. Discuss the cloning of plasmids, vectors, DNA, RNA, cDNA, proteins, and other molecules for expression, sub-cloning, and other methods. Also discuss digestion, ligation, transformation, plasmid preparation, cDNA library screening, and competent cells here.


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Old 04-16-2009, 03:22 PM
Pipette Filler
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Default signal peptide



Does anyone know if E.coli can recognize mammalian signal peptides?. I cloned a mammalian protein with its signal peptide but I donīt see any expression in E. coli. Could it be possible it is been secreted to the media?.

Thanks for your time.
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