A technician recently made a mistake by adding way to much insert during a ligation-transformation procedure. The ratio was nearly 150:1 insert to vector instead of customary 4:1 ratio. The plate resulting plate did not produce any viable colonies. Members of the lab found this quite interesting and prepared several dilutions ranging from 1:1 to 100:1 and found that indeed when too much insert to vector or too little insert to vector produced fewer colonies with a normal amount of colonies appearing between 3:1 and 40:1.
We came up with a few ideas as to why this may be the case biologically.
Does anyone know anything about the cause of this or were we can look?