This is a follow up to my dead E. coli
problem (posted already on the forum).
I have been using a kit from Clontech called in-fusion 2.0 for cloning, produced my clones and transformed them into their "super competent" FusionBlue E. coli
cells. This worked fine and produced many colonies, but I am keen to get back to using a more common generic cloning strain used in our lab already, or common in other labs.
I have tried to transform the same clones into DH5 alpha cells, but no colonies grew. For these reactions you need "super competent" cells (according to the website), with a transformation efficiency of greater than 10^8 cfu/ug DNA (that doesn't mean anything to me, but if it does to anyone else?....).
I was wondering if anyone could suggest any strains I could try, I've been told to try Invitrogen's Top10 E. coli
or Excel-1-Blue/NovaBlue (Transformation efficiency: 10^9 cfu/μg DNA).
Are there any others which could be useful?