|Register||Search||Today's Posts||Mark Forums Read|
|Molecular Cloning Forum DNA cloning forum. Discuss the cloning of plasmids, vectors, DNA, RNA, cDNA, proteins, and other molecules for expression, sub-cloning, and other methods. Also discuss digestion, ligation, transformation, plasmid preparation, cDNA library screening, and competent cells here.|
| ||LinkBack||Thread Tools||Display Modes|
E. coli strains
This is a follow up to my dead E. coli problem (posted already on the forum).
I have been using a kit from Clontech called in-fusion 2.0 for cloning, produced my clones and transformed them into their "super competent" FusionBlue E. coli cells. This worked fine and produced many colonies, but I am keen to get back to using a more common generic cloning strain used in our lab already, or common in other labs.
I have tried to transform the same clones into DH5 alpha cells, but no colonies grew. For these reactions you need "super competent" cells (according to the website), with a transformation efficiency of greater than 10^8 cfu/ug DNA (that doesn't mean anything to me, but if it does to anyone else?....).
I was wondering if anyone could suggest any strains I could try, I've been told to try Invitrogen's Top10 E. coli or Excel-1-Blue/NovaBlue (Transformation efficiency: 10^9 cfu/μg DNA).
Are there any others which could be useful?
Last edited by niki_nje; 02-13-2009 at 05:07 PM.
|coli , strains|
|Thread||Thread Starter||Forum||Replies||Last Post|
|differences between E. coli strains Top10 and DH10B||fierem||Protocols and Methods Forum||3||11-30-2010 12:24 AM|
|Plasmid DNA Isolation from E. Coli?||ying||DNA Techniques||0||07-28-2009 05:16 PM|
|E. coli electroporation efficiencies - pBR322 v/s pUC ori||Haviland, David L||Protocols and Methods Forum||3||06-26-2006 03:18 AM|
|E. coli with GFP||Subhash Kulkarni||Microbiology Forum||0||09-09-2003 10:56 AM|
|Standard S pombe strains||SLF||Yeast Forum||0||07-15-2003 07:50 PM|