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Electroporation not working, need help
I am trying to insert the plasmid using electroporation in gram negetive bacteria ( many of my lab mates have done in past).. Its only me. My electro-competent cells are fine as when I spead them on regular LB plate (no antibiotic), they are growing..
Now, I use single pulse, Use SOC as nutrient medium after giving pulse for recovery. My recovery time is 1hr. I use 0.1cm gap cuvette.
what steps can be done differently to trouble shoot? should i give longer pulse/ double pulse....
|electroporation , working|
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