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Molecular Cloning Forum DNA cloning forum. Discuss the cloning of plasmids, vectors, DNA, RNA, cDNA, proteins, and other molecules for expression, sub-cloning, and other methods. Also discuss digestion, ligation, transformation, plasmid preparation, cDNA library screening, and competent cells here.


Blue White Cloning Selection Positive Colonies

Blue White Cloning Selection Positive Colonies - Molecular Cloning Forum

Blue White Cloning Selection Positive Colonies - DNA cloning forum. Discuss the cloning of plasmids, vectors, DNA, RNA, cDNA, proteins, and other molecules for expression, sub-cloning, and other methods. Also discuss digestion, ligation, transformation, plasmid preparation, cDNA library screening, and competent cells here.


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Old 02-20-2008, 02:30 AM
Pipette Filler
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Question Blue White Cloning Selection Positive Colonies



Hi i hate selecting hundred clones for 1 positive clone.

How do you guys do the blue/white colony selection with lac-Z (beta-gal)?

I want to use x-gal for this but have never done this myself. My plasmid has the blue and white selection for this.

cheers
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Old 02-20-2008, 09:45 AM
Pipette Filler
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Default Re: Blue White Cloning Selection Positive Colonies

Yeah Use X gal on you plates before you plate your tranformed cells.. white ones are "supposed" to be positive... But sometimes even blue colonies are positive and white one negetive... so you will have to screen many colonies to get a positive clone... But.. It would definately not 100...
All the best!!!
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