Antarctic Phosphatase DNA Cloning

dnamolecule · #1 (**permalink**) 01-23-2008, 04:04 AM

Just saw the other post on Antarctic Phosphatase!

Antarctic Phosphatase rocks. It works VERY QUICKLY for dephosphorylation.

I used it many times and it is much better than SAP or CIP.

You can get it from NEB.

JO18 · #2 (**permalink**) 03-24-2008, 02:03 PM

Hi,
I've used the Antarctic Phosphatase from NEB but I've had lots of clones in negatif control ligation.
Could u plz tell me the conditions for the dephosphorylation that u have used for ur cloning.
Thnx

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Antarctic Phosphatase DNA Cloning

Molecular Cloning Forum

DNA cloning forum. Discuss the cloning of plasmids, vectors, DNA, RNA, cDNA, proteins, and other molecules for expression, sub-cloning, and other methods. Also discuss digestion, ligation, transformation, plasmid preparation, cDNA library screening, and competent cells here.