Isolating Large DNA Vectors Without CsCl

malabi · #1 (**permalink**) 01-17-2008, 07:50 PM

I am looking for a good method to isolate large (215 kb DNA) without resorting to old-fashioned CsCl centrifugation. I have tried some of the methods used for BAC/PAC purifications but there must be some trick because I don't get very good DNA. Any suggestions?

admin · #2 (**permalink**) 01-23-2008, 04:21 AM

Hello Malabi,

there are many kits for large DNA vector purification.

Here is a protocol using gel filtration:
SpringerLink - Journal Article

Has anyone heard of ProPrep Kits? They are used for large BAC and large plasmid purification.

CsCl gradients are still the gold standard I think for large plasmid isolations / purifications.

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Isolating Large DNA Vectors Without CsCl

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