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| Molecular Cloning Forum DNA cloning forum. Discuss the cloning of plasmids, vectors, DNA, RNA, cDNA, proteins, and other molecules for expression, sub-cloning, and other methods. Also discuss digestion, ligation, transformation, plasmid preparation, cDNA library screening, and competent cells here. |
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01-24-2008, 10:15 PM
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 problem with cloning/restriction digest I am trying to clone an insert into a Pet-30 vector. I generated the insert by PCR adding on NdeI/XhoI ends. I cleaned up my pcr using the Qiaquick PCR purification. I digested my product (using Nde/XhoI) for about 3 hrs @37 deg. When I run the digest on a gel, I see my insert (which appears to be the right size), but not the vector. Help?        |
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