| |||||||
| Register | Blogs | FAQ | Members List | Calendar | Search | Today's Posts | Mark Forums Read |
| Molecular Cloning Forum DNA cloning forum. Discuss the cloning of plasmids, vectors, DNA, RNA, cDNA, proteins, and other molecules for expression, sub-cloning, and other methods. Also discuss digestion, ligation, transformation, plasmid preparation, cDNA library screening, and competent cells here. |
Isolating Large DNA Vectors Without CsCl
Molecular Cloning Forum
DNA cloning forum. Discuss the cloning of plasmids, vectors, DNA, RNA, cDNA, proteins, and other molecules for expression, sub-cloning, and other methods. Also discuss digestion, ligation, transformation, plasmid preparation, cDNA library screening, and competent cells here.
 |
 |
| | LinkBack | Thread Tools | Display Modes |
01-17-2008, 07:50 PM
| |||||
| |||||
 Isolating Large DNA Vectors Without CsCl I am looking for a good method to isolate large (215 kb DNA) without resorting to old-fashioned CsCl centrifugation. I have tried some of the methods used for BAC/PAC purifications but there must be some trick because I don't get very good DNA. Any suggestions?        |
| Today
| ||||
| ||||
 Sponsored Links |
|
01-23-2008, 04:21 AM
| ||||||
| ||||||
 Re: Isolating Large DNA Vectors Without CsCl Hello Malabi, there are many kits for large DNA vector purification. Here is a protocol using gel filtration: SpringerLink - Journal Article Has anyone heard of ProPrep Kits? They are used for large BAC and large plasmid purification. CsCl gradients are still the gold standard I think for large plasmid isolations / purifications. |
Linear Mode
