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Double Ligation Reaction

Molecular Cloning Forum DNA cloning forum. Discuss the cloning of plasmids, vectors, DNA, RNA, cDNA, proteins, and other molecules for expression, sub-cloning, and other methods. Also discuss digestion, ligation, transformation, plasmid preparation, cDNA library screening, and competent cells here.

Double Ligation Reaction

Molecular Cloning Forum

DNA cloning forum. Discuss the cloning of plasmids, vectors, DNA, RNA, cDNA, proteins, and other molecules for expression, sub-cloning, and other methods. Also discuss digestion, ligation, transformation, plasmid preparation, cDNA library screening, and competent cells here.

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01-06-2008, 07:24 AM

deebee12

Pipette Filler

Join Date: Feb 2006

Posts: 9

Double Ligation Reaction

Hi
has anyone ever carried out a double ligation reaction successfully?

Any tips on this?

thanks guys

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« QIAprep or QIAquick columns? | DNA Ligation Problem Double Digestion »

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Molecular Biology Forums

Double Ligation Reaction

Molecular Cloning Forum

DNA cloning forum. Discuss the cloning of plasmids, vectors, DNA, RNA, cDNA, proteins, and other molecules for expression, sub-cloning, and other methods. Also discuss digestion, ligation, transformation, plasmid preparation, cDNA library screening, and competent cells here.