		Molecular Biology Forum > Molecular Research Topics Forum > Molecular Biology Techniques > Molecular Cloning Forum
Double Ligation Reaction

Molecular Cloning Forum DNA cloning forum. Discuss the cloning of plasmids, vectors, DNA, RNA, cDNA, proteins, and other molecules for expression, sub-cloning, and other methods. Also discuss digestion, ligation, transformation, plasmid preparation, cDNA library screening, and competent cells here.

transformation Videos

· · ·
bacterial-dna-ligation-transformation Video

· · ·
dna-electroporation-ligation Video

Double Ligation Reaction

Molecular Cloning Forum

DNA cloning forum. Discuss the cloning of plasmids, vectors, DNA, RNA, cDNA, proteins, and other molecules for expression, sub-cloning, and other methods. Also discuss digestion, ligation, transformation, plasmid preparation, cDNA library screening, and competent cells here.

LinkBack

Thread Tools

Display Modes

#1 (permalink)

01-06-2008, 07:24 AM

deebee12

Pipette Filler

Join Date: Feb 2006

Posts: 11

Double Ligation Reaction

Hi
has anyone ever carried out a double ligation reaction successfully?

Any tips on this?

thanks guys

Today

Advertising
Google Adsense

This advertising will not be shown

in this way to registered members.

and become a member on

Molecular Biology Forum

Sponsored Links

Tags

dna, ligation

« QIAprep or QIAquick columns? | DNA Ligation Problem Double Digestion »

Thread Tools
Show Printable Version Email this Page
Display Modes
Linear Mode Switch to Hybrid Mode Switch to Threaded Mode

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is Off Trackbacks are On Pingbacks are On Refbacks are On

Forum Jump

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
Blunt Ligation Reaction	Azzy	Molecular Biology Techniques	1	04-25-2008 08:16 AM
DNA Ligation Problem Double Digestion	Denise	Molecular Cloning Forum	0	01-09-2008 01:41 PM
calculate volume of buffer in reaction mixture	spicooli	Biochemistry Forum	2	10-22-2007 03:36 AM
PCR and Topo Cloning	kiki06	PCR - Polymerase Chain Reaction Forum	2	12-05-2006 09:49 PM
Polymerase Chain Reaction - lab for students	domba	Molecular Biology Articles and Protocols	0	07-31-2006 11:02 PM

All times are GMT. The time now is 10:39 AM.

Member Login

Double Ligation Reaction

Molecular Cloning Forum

DNA cloning forum. Discuss the cloning of plasmids, vectors, DNA, RNA, cDNA, proteins, and other molecules for expression, sub-cloning, and other methods. Also discuss digestion, ligation, transformation, plasmid preparation, cDNA library screening, and competent cells here.