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Antibodies for ELISA

Antibodies for ELISA - Molecular Biology Techniques

Antibodies for ELISA - Molecular Biology Forum. Includes forums for common molecular biology techniques.


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Old 05-02-2014, 12:41 PM
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Question Antibodies for ELISA



I have some really basic questions as I have never done an ELISA (or western blot) before.

I need to detect beta-lactoglobulin in cow's milk with sandwich ELISA and am not sure what antibodies to use.

Are rabbit anti-bovine beta-lactoglobulin polyclonal antibody (primary antibody) & goat anti-rabbit IgG antibody peroxidase conjugated (secondary antibody) alright?

Also, is 50mM Tris, 0.14M NaCl, 0.05% Tween 20, pH 8.0 okay as blocking soution for this assay? Or is it better to use 3% ovalbumin in PBS? Is Tween essential for blocking?

Would really appreciate some advice.

Thanks
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