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Monolayer on 24-well transwell insert

Monolayer on 24-well transwell insert - Molecular Biology Techniques

Monolayer on 24-well transwell insert - Molecular Biology Forum. Includes forums for common molecular biology techniques.


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Old 08-15-2013, 02:02 PM
Pipette Filler
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Default Monolayer on 24-well transwell insert



We are having difficulties getting a confluent HUVEC monolayer on a 24-well transwell insert. I have tried different dilutions of collagen (1:20 dilution and a 1:5 dilution from 20x rat tail collagen) as well as the amount of cells seeded onto the insert (2x10^5 and 4x10^5). We have reach confluency in the middle of the insert, but the edges we have gaps between the cells. Does anyone have any suggestions?
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Old 08-22-2013, 01:07 PM
Pipette Filler
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Default Re: Monolayer on 24-well transwell insert

Hi Vogelmn,

My name is Hilary Sherman and I am an Applications Scientist for Corning. I have a lot of experience with Transwells and I would like to see if I can help. Please email me at [Only registered users see links. ].

Best,
Hilary
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24well , insert , monolayer , transwell


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