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Calculation of a dilution factor??

Calculation of a dilution factor?? - Molecular Biology Techniques

Calculation of a dilution factor?? - Molecular Biology Forum. Includes forums for common molecular biology techniques.


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Old 08-01-2013, 11:02 AM
Pipette Filler
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Default Calculation of a dilution factor??



Hi all.

One question...

I have to calculate the dilution factor of serum in my preparation to be able to calculate the final concentration in a HPLC setup.

This is the prep:

25 ul serum mixed with 5 ul solvent + 1000 ul AcN (to precipitate proteins)

vortex

pippette 995 ul into new epp. and evaporate to dryness in speedvac

reconstitute with 100 ul solvent

How does one calculate the dilution factor??

Thanks
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