Please help! I am new to gel electrophoresis and I keep stumbling into different problems and have no one to help.
I am having issues with reduced sensitivity down my gel. I have been using a mini gel and splitting it into 3 so that I can process more samples at a time but the bottom portion is not as sensitive. I also seem to be struggling to get the ladders on the right hand side running correctly.
I have tried running 2% agarose run at 100V for 60 mins, and 3% agarose run at 130V for 60 mins. I put EtBr in my gel before I pour it. And I use the same 1x TBE buffer to make the gel and as a running buffer. I have tried using different ladders and I have also tried rotating the gel on the light box in case that was a problem.
I have tried everything I can think of and running out of ideas.... Please help me, I am loosing precious time on my research.