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Reduced sensitivity down the gel

Reduced sensitivity down the gel - Molecular Biology Techniques

Reduced sensitivity down the gel - Molecular Biology Forum. Includes forums for common molecular biology techniques.


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Old 07-03-2013, 05:48 PM
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Unhappy Reduced sensitivity down the gel



Please help! I am new to gel electrophoresis and I keep stumbling into different problems and have no one to help.

I am having issues with reduced sensitivity down my gel. I have been using a mini gel and splitting it into 3 so that I can process more samples at a time but the bottom portion is not as sensitive. I also seem to be struggling to get the ladders on the right hand side running correctly.

I have tried running 2% agarose run at 100V for 60 mins, and 3% agarose run at 130V for 60 mins. I put EtBr in my gel before I pour it. And I use the same 1x TBE buffer to make the gel and as a running buffer. I have tried using different ladders and I have also tried rotating the gel on the light box in case that was a problem.

I have tried everything I can think of and running out of ideas.... Please help me, I am loosing precious time on my research.
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