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Protocol for reprobing with the SAME antibody after no bands.

Protocol for reprobing with the SAME antibody after no bands. - Molecular Biology Techniques

Protocol for reprobing with the SAME antibody after no bands. - Molecular Biology Forum. Includes forums for common molecular biology techniques.


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Old 06-22-2013, 11:27 PM
Pipette Filler
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Default Protocol for reprobing with the SAME antibody after no bands.



I've been having issues with the one antibody which has worked in previous studies on the same fish.

I wanted to incubate longer with the primary but I have already imaged with ECL. If I wanted to try again, can I wash off the ECL (3x 10 mins 1xTTBS), reblock (1 hr 5% dry milk in 1X TTBS), and reincubate with primary for longer (overnight at 4C)? Can blocking damage the membrane? How long are proteins stable in a membrane? Can washing damage the membrane?
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