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  #1  
Old 05-14-2013, 01:22 PM
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Hellow everybody!
I'am trying to separate two proteines (280 and 290 kDa), but untill now the resolution is not significant, which voltage do you think is better to separate thesse two proteins using a 3-8 % PAGE?
I would be gratreful if you could help me
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Old 06-04-2013, 11:09 AM
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Default Re: Sds-page

any news stillhere?

Btw, what gel system are you using? big or small? If your gel size is small i figure it would be difficult to get that resolution. But I shall wait for your reply first.
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Old 06-05-2013, 02:34 PM
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Hi,
Actually we use small gels, and we couldn't separate the two proteins with the same antibody, so we are waiting until we get the specific antibody against the protein 290 kDa to find out if there is realy the mutated protein.
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Old 06-10-2013, 11:31 AM
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Default Re: Sds-page

try look at this paper maybe can help
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Old 02-15-2014, 11:47 AM
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Default Re: Sds-page

You should use an appropriate pre-stained marker and run the electrophoresis until you Mw area of interest is separated enough. This may mean letting proteins as large as >100kDa go out of the gel.
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