| | Re: RFLP troubleshooting
For what I see I don't think decreasing gel electrophoresis time should be wise. Actually you might as well increasing it a little in order to have better resolution of your ladder.
The gel looks very good. I can see what you say about smaller fragments (faint signal), except for the lowest, I presume it is an artifact like dimerized primers. You say your fragments are between 100-1000 bp but I'm seeing two bands below 100 bp in lanes 4 and 5, were you specting them?
As I said before, the gel looks good. I don't see any problem with your technique. The only thing I can tell you to do to improve further is to load more sample per well. Difference between large and small fragments will remain but at least you will have a brighter signal.
I hope this has help you. Good luck Gislab
Last edited by luisillo; 05-09-2013 at 01:25 PM.