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Log phase and lag phase question?

Log phase and lag phase question? - Molecular Biology Techniques

Log phase and lag phase question? - Molecular Biology Forum. Includes forums for common molecular biology techniques.


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Old 05-02-2013, 01:01 AM
Pipette Filler
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Question Log phase and lag phase question?



Hi there, I'm a nursing student and I got this assignment that I have a question about..For the record my teacher is a douche canoe and his instructions don't help at all one bit. Anyone interested in enlightening me as to the what the lag and log phase is? I'm pretty sure I'm mistaken.. I was wondering if you guys could give me a viable definition of log and lag phase and how to identify them? Here's the graph I've made so far if it helps?
Forgive me if this has been answered already, I work third shift and need to get this done asap. I'm not asking for answers mind you, I'm just asking for the definitions.. If anyone can help me out I'd be much appreciative!

Here's the math I have so far for the generation time. Which I just did in my own way:P

.460 - .187 = .273

.273/4 = .06825

.06825 + .167= .25525

.460 - .06825 = .39175
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Last edited by L Jo; 05-02-2013 at 01:02 AM.
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Old 05-04-2013, 02:41 PM
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Default Re: Log phase and lag phase question?



Hi L Jo
the Lag phase is non-exponential growth, the time time before exponential growth where bacteria are synthesizing enzymes, proteins and getting ready to divide.

It seems its 0-60 min.

The Log Phase or Exponential Phase exhibit exponential growth (doubling) or log increase in number.
The is after 60 minutes to 120-140 minutes, and Log phase or exponential transitions then to the stationary phase.

This phase is 140-180 minutes.

The amount of light transmitted through a sample is inversely proportional to cell number. So as transmitance is reduced as graphed above (black line) the bacteria increase as per discussion above.

To learn more about the relationship of Transmitance and Absorbance to cell number and counts see

http://inst.bact.wisc.edu/inst/index...cle&art_id=112

and

http://inst.bact.wisc.edu/inst/index...cle&art_id=107
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bacteria , growth curve , lag , log , phase , question


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