Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Molecular Biology Techniques
Register Search Today's Posts Mark Forums Read

Molecular Biology Techniques Molecular Biology Forum. Includes forums for common molecular biology techniques.


EBV internal control

EBV internal control - Molecular Biology Techniques

EBV internal control - Molecular Biology Forum. Includes forums for common molecular biology techniques.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 04-02-2013, 05:24 AM
Pipette Filler
Points: 565, Level: 11 Points: 565, Level: 11 Points: 565, Level: 11
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Jul 2012
Location: Muscat .Oman
Posts: 1
Thanks: 0
Thanked 0 Times in 0 Posts
Post EBV internal control



hi there

background
well iam a lab technician wroking in a clinical diagnosis labortories, mainley in virology, where we dignose viruses. one o fthe viruses that we detect is EBV. we have used Abott EBV real-time pcr kit as reagents. for the last 2 years we have a problem with the kit internal control. we have discuused this isse with company and even though they tried to help but the problem is not solved yet.

the problem

the internal control is not working in the sample , as there is no amplification curve in real-time is showing, no CT value.
the internal control is working with kits standards.

we include the internal control in the sample DNA purfification step and in PCR reagent prepartion for standards and NTC.


Question
so what could be the problem??
why the IC is working with standards not the sample??
Reply With Quote
Reply


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Internal Control slmetzger PCR - Polymerase Chain Reaction Forum 2 10-15-2010 02:55 PM
internal control of western blot for E. coli lattee Western Blot Forum 0 07-30-2009 03:33 AM
Internal Amplification Control in Real time PCR Aga Real-Time PCR and Quantitative PCR Forum 1 05-23-2009 02:55 AM
internal control for plant proteins in WB izacil Western Blot Forum 1 04-09-2008 02:21 PM
primers for internal control of RT-PCR in yeast josmarlangner@yahoo.de Protocols and Methods Forum 0 05-13-2005 02:06 PM


All times are GMT. The time now is 10:11 AM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.11105 seconds with 16 queries