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EBV internal control

EBV internal control - Molecular Biology Techniques

EBV internal control - Molecular Biology Forum. Includes forums for common molecular biology techniques.

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Old 04-02-2013, 05:24 AM
Pipette Filler
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Post EBV internal control

hi there

well iam a lab technician wroking in a clinical diagnosis labortories, mainley in virology, where we dignose viruses. one o fthe viruses that we detect is EBV. we have used Abott EBV real-time pcr kit as reagents. for the last 2 years we have a problem with the kit internal control. we have discuused this isse with company and even though they tried to help but the problem is not solved yet.

the problem

the internal control is not working in the sample , as there is no amplification curve in real-time is showing, no CT value.
the internal control is working with kits standards.

we include the internal control in the sample DNA purfification step and in PCR reagent prepartion for standards and NTC.

so what could be the problem??
why the IC is working with standards not the sample??
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