Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Molecular Biology Techniques
Register Search Today's Posts Mark Forums Read

Molecular Biology Techniques Molecular Biology Forum. Includes forums for common molecular biology techniques.


Rebeca Castro

Rebeca Castro - Molecular Biology Techniques

Rebeca Castro - Molecular Biology Forum. Includes forums for common molecular biology techniques.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 04-01-2013, 11:26 PM
Pipette Filler
Points: 243, Level: 4 Points: 243, Level: 4 Points: 243, Level: 4
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Jan 2013
Posts: 3
Thanks: 0
Thanked 0 Times in 0 Posts
Question Rebeca Castro



How long do you stain with Ethidium Bromide a DGGE gel when running PCR 16S
rRNA V9? I ran the gel for 19 hours at 50 V. Thanks
Reply With Quote
  #2  
Old 04-02-2013, 03:12 PM
luisillo's Avatar
M.D/Ph.D
Points: 3,877, Level: 41 Points: 3,877, Level: 41 Points: 3,877, Level: 41
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Jul 2010
Location: Mexico
Posts: 352
Thanks: 19
Thanked 98 Times in 90 Posts
Default Re: Rebeca Castro

Standarize it. You could try a gradient with the same gel: stain it for a short time first and see the results. If it was not enough you can stain it for another short time. Repeat it until you have th desired result. Do not forget to photograph the gel each time you see it with UV light (in a UV transiluminator) so that in case the etBr screws the gel you already have a picture of you results.

You can also try silver staining. I'm not sure how convinient is with DGGE gel, but I've read is the best for TGGE gel staining.

Good luck
Reply With Quote
  #3  
Old 05-06-2013, 04:22 PM
Pipette Filler
Points: 22, Level: 1 Points: 22, Level: 1 Points: 22, Level: 1
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: May 2013
Posts: 1
Thanks: 0
Thanked 0 Times in 0 Posts
Default Re: Rebeca Castro

good luck
Reply With Quote
Reply

Tags
castro , rebeca


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Rebeca Castro rebe60 Molecular Biology Techniques 0 02-03-2013 06:57 PM
Rebeca Castro rebe60 New Member Introductions Forum 2 01-05-2013 06:06 AM


All times are GMT. The time now is 09:41 AM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.12030 seconds with 16 queries