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Transfer time and voltage conditions for Bolt and mahoney blotting buffer

Transfer time and voltage conditions for Bolt and mahoney blotting buffer - Molecular Biology Techniques

Transfer time and voltage conditions for Bolt and mahoney blotting buffer - Molecular Biology Forum. Includes forums for common molecular biology techniques.


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Old 03-16-2013, 06:16 PM
Pipette Filler
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Default Transfer time and voltage conditions for Bolt and mahoney blotting buffer



Hi all,

Was wondering about the transfer conditions (time, current and voltage) for the bolt and mahoney transfer buffer. The book and the original paper recommends 10V for 2h. Is it advisable to do this for 10V and overnight? Thank you~
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