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qRT-PCR of miRNA

qRT-PCR of miRNA - Molecular Biology Techniques

qRT-PCR of miRNA - Molecular Biology Forum. Includes forums for common molecular biology techniques.

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Old 03-16-2013, 06:23 AM
Pipette Filler
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Default qRT-PCR of miRNA

hi, i am trying to quantify miRNA by qRT PCR BioRad, but unfortunately i get only noise,, no signal. what can be the possibilities?? how can i sort it out??
i isolate RNA from human serum with trizol method, then i do poly A tail polymerase reaction then reverse trancription reaction. i use SYBER GREEn for qRT.
i didnot quantify the RNA for PAP, RT reaction.

can you suggest any sucessful manual protocol for bisulphite conversion of DNA??? i used CpG modification kit, Millipore but that did not worked.
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mirna , qrtpcr

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