hi, i am trying to quantify miRNA by qRT PCR BioRad, but unfortunately i get only noise,, no signal. what can be the possibilities?? how can i sort it out??
i isolate RNA from human serum with trizol method, then i do poly A tail polymerase reaction then reverse trancription reaction. i use SYBER GREEn for qRT.
i didnot quantify the RNA for PAP, RT reaction.
can you suggest any sucessful manual protocol for bisulphite conversion of DNA??? i used CpG modification kit, Millipore but that did not worked.