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Leupeptin and pepstatin impact polyribosome distribution-differential centrifugationn
I have been doing cellular extractions with lysis buffer (mild detergent) followed by differential centrifugations and found that when I switched to using leupeptin/pepstatin at10X I obtained all polyribosomes in P2 pellet with cytoskeleton and nucleus instead of the usual P100 (polyribosomal) fraction. Why would this happen? Does anyone know what is going on? Thanks ahead of time!
Last edited by melody_of_sorrow; 02-19-2013 at 05:10 PM. Reason: Forgot a detail
|centrifugationn , distributiondifferential , impact , leupeptin , lysis polyribosome , pepstatin , polyribosome|