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Western blotting- sparkly dots

Western blotting- sparkly dots - Molecular Biology Techniques

Western blotting- sparkly dots - Molecular Biology Forum. Includes forums for common molecular biology techniques.


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  #1  
Old 02-02-2013, 12:35 AM
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Default Western blotting- sparkly dots



Hi all,

This the first time I've done a western blot. when placed for detection in UVP, some how all these bright spots (on the dark background of the blot) appear. are these bubbles from the wrap? Please let me know. Thanks!
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Old 02-04-2013, 07:20 AM
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Default Re: Western blotting- sparkly dots

Can you please attach a picture for us to view? or link it somewhere. western blot with UVP? Or do you mean flourescene label?
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Old 03-04-2013, 07:59 PM
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Default Re: Western blotting- sparkly dots

I meant the western blot (supersignal western pico chemiluminiscent substrate) detection. Not the fluoroscence.
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Old 03-05-2013, 02:19 AM
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Default Re: Western blotting- sparkly dots

It seem like fiber or etc. Just clean the surface of the uvp before take image again.
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Old 03-05-2013, 04:44 PM
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Default Re: Western blotting- sparkly dots



Hi and welcome, looks like either noise from image processing or high signal due to a dirty blot (from HRP - lum).
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