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peace upon you

peace upon you - Molecular Biology Techniques

peace upon you - Molecular Biology Forum. Includes forums for common molecular biology techniques.


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Old 01-25-2013, 08:31 AM
Pipette Filler
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im working on 3 human gens about ( 200 ,300 , 400 )bp .

my samples genamic DNA from blood .

my supervisor dilut the primers to 100 pmol.

i use Accua pwoer primix = 5 microliter

total reaction volume = 20 microliter ( 9 microliter DNA ,, 1 microliter primers )

program : 95 c ..5min

95 c .. 1min
55 c ..1min
72 c ..1min

72 c .. 7 min

35 cycles


at first the pcr is ok and the product perfect .... then primer dimer result and no product . what's going on ???
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