Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Molecular Biology Techniques
Register Search Today's Posts Mark Forums Read

Molecular Biology Techniques Molecular Biology Forum. Includes forums for common molecular biology techniques.


Immunofluorescence co-labelling problem

Immunofluorescence co-labelling problem - Molecular Biology Techniques

Immunofluorescence co-labelling problem - Molecular Biology Forum. Includes forums for common molecular biology techniques.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 01-23-2013, 07:43 PM
Pipette Filler
Points: 234, Level: 4 Points: 234, Level: 4 Points: 234, Level: 4
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Jan 2013
Location: Midwest
Posts: 4
Thanks: 0
Thanked 0 Times in 0 Posts
Exclamation Immunofluorescence co-labelling problem



Hello,
I want to do a co-labelling immunofluorescence experiment on mouse parafin-embedded tissue sections. The first primary antibody is a Anti-X raised in rabbit, IgG fraction and the second primary is Anti-Y raised in mouse. Since I know there can be issues with background staining using mouse monoclonal antibodies in mouse tissues, I'm not sure how I can account for this in my double staining protocol. I have optimized the protocol for Anti-X using a goat anti-rabbit secondary but haven't tried the mouse Anti-Y antibody yet since the tissue sections I have are valuable and can't be wasted. Can someone help with ideas/protocol to double stain with these primaries?

Thanks,
Reply With Quote
Reply

Tags
antibody problems , colabelling , immunofluorescence , mouse on mouse staining , problem


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Immunofluorescence Problem DrIHC Molecular Biology Techniques 0 02-16-2012 05:13 PM
Immunofluorescence Problem DrIHC General Science Questions and Layperson Board 0 02-16-2012 05:05 PM
SDS-PAGE problem Steve Nothwehr Protocols and Methods Forum 2 10-16-2009 08:59 AM
N-body problem Torstein Raanes Physics Forum 1 12-01-2003 10:31 AM
Integration Problem / Finding Contour lines Problem blah12@mail.com Physics Forum 2 09-07-2003 11:28 PM


All times are GMT. The time now is 06:46 PM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.11038 seconds with 16 queries