Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Molecular Biology Techniques
Register Search Today's Posts Mark Forums Read

Molecular Biology Techniques Molecular Biology Forum. Includes forums for common molecular biology techniques.

PCR non specific band

PCR non specific band - Molecular Biology Techniques

PCR non specific band - Molecular Biology Forum. Includes forums for common molecular biology techniques.

LinkBack Thread Tools Display Modes
Old 01-08-2013, 06:43 PM
Pipette Filler
Points: 1,135, Level: 19 Points: 1,135, Level: 19 Points: 1,135, Level: 19
Activity: 0% Activity: 0% Activity: 0%
Join Date: Jul 2011
Posts: 4
Thanks: 0
Thanked 0 Times in 0 Posts
Default PCR non specific band

I have done a gradient PCR for my PCR reaction. I m doing this reaction with cDNA obtained from a cell line and Bioline mastermix
First well annealing temp- 60
second well- 57
4th well - 55
marker - 100bp

The problem is I m getting my PCR product which is 682bp bt along with that product I do get non specific bands. My primer tm is 58

Though I have tried gradient I m not still getting a single band product ?
Attached Images
File Type: jpg pcr hras 8 1 2013 repeat with pcr product.JPG (20.7 KB, 0 views)
Reply With Quote

band , pcr , specific

Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
nice tight and dark non specific band Tfal Western Blot Forum 0 05-15-2009 04:52 AM
Calculations for specific activity Morales, Victor Protein Forum 0 10-02-2008 01:17 PM
white sample band but black positive control band wilairat Western Blot Forum 6 01-27-2008 01:13 AM
no amplification or non specific band yucel PCR - Polymerase Chain Reaction Forum 3 03-24-2007 03:54 PM
combining Conduction Band theory with Electronegativity as one phenomenon Archimedes Plutonium Chemistry Forum 2 10-12-2003 06:25 PM

All times are GMT. The time now is 11:44 PM.

Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2015, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.12357 seconds with 17 queries