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PCR non specific band

PCR non specific band - Molecular Biology Techniques

PCR non specific band - Molecular Biology Forum. Includes forums for common molecular biology techniques.


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Old 01-08-2013, 06:43 PM
Pipette Filler
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Default PCR non specific band



I have done a gradient PCR for my PCR reaction. I m doing this reaction with cDNA obtained from a cell line and Bioline mastermix
First well annealing temp- 60
second well- 57
4th well - 55
marker - 100bp

The problem is I m getting my PCR product which is 682bp bt along with that product I do get non specific bands. My primer tm is 58

Though I have tried gradient I m not still getting a single band product ?
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