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#1
01-08-2013, 05:43 PM
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 PCR non specific band I have done a gradient PCR for my PCR reaction. I m doing this reaction with cDNA obtained from a cell line and Bioline mastermix First well annealing temp- 60 second well- 57 4th well - 55 marker - 100bp The problem is I m getting my PCR product which is 682bp bt along with that product I do get non specific bands. My primer tm is 58 Though I have tried gradient I m not still getting a single band product ?  |
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| band , pcr , specific |
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