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Invitrotranscription

Invitrotranscription - Molecular Biology Techniques

Invitrotranscription - Molecular Biology Forum. Includes forums for common molecular biology techniques.


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Old 01-01-2013, 08:55 AM
Pipette Filler
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Default Invitrotranscription



hi
I am facing problem when i try to amplify cDNA from invitrotranscribed RNA. the problem is i use to get good yeild of invitrotranscribed RNA almost 3000 ng/ul on nanodrop and good 260/230 ratio but the band is not visible on gel. when i processed the RNA FOR cDNA synthesis i got good DNA too.bt it is nt getting amplified,the transcript size is 885 bp.
Kindly tell me the probable errors i must have made
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