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smitarao 01-01-2013 08:55 AM

Invitrotranscription
 
hi
I am facing problem when i try to amplify cDNA from invitrotranscribed RNA. the problem is i use to get good yeild of invitrotranscribed RNA almost 3000 ng/ul on nanodrop and good 260/230 ratio but the band is not visible on gel. when i processed the RNA FOR cDNA synthesis i got good DNA too.bt it is nt getting amplified,the transcript size is 885 bp.
Kindly tell me the probable errors i must have made


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