I am facing problem when i try to amplify cDNA from invitrotranscribed RNA. the problem is i use to get good yeild of invitrotranscribed RNA almost 3000 ng/ul on nanodrop and good 260/230 ratio but the band is not visible on gel. when i processed the RNA FOR cDNA synthesis i got good DNA too.bt it is nt getting amplified,the transcript size is 885 bp.
Kindly tell me the probable errors i must have made
|All times are GMT. The time now is 02:04 AM.|
Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved