I am currently trying to culture suspension monomac-6 cells (monocytic). They appear to be increasing in number under the microscope however when counting them they continually seem to drastically reduce. As the coutler counter bases counts on particles within solution I am not sure whether it will register dead cells in the counts. If it does count dead cells then I am perplexed as to where these cells are disappearing to. I have checked the centrifuge speed and I dont think its that.
If anyone has had any experience culturing these cells or have any idea where I am going wrong it would be much appreciated!