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-   -   unwanted deletion in cloning (http://www.molecularstation.com/forum/molecular-biology-techniques/86829-unwanted-deletion-cloning.html)

Jimenita 11-19-2012 12:16 AM

unwanted deletion in cloning
 
I am trying to clone a 2000 pb fragment into a proviral-vector and I got colonies (stbl2) but when I screen them they are deletionated...
Suggestions? Medium?
Thanks!!!


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