Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Molecular Biology Techniques
Register Search Today's Posts Mark Forums Read

Molecular Biology Techniques Molecular Biology Forum. Includes forums for common molecular biology techniques.


Problems in PCR "microsatellites" trubleshooting

Problems in PCR "microsatellites" trubleshooting - Molecular Biology Techniques

Problems in PCR "microsatellites" trubleshooting - Molecular Biology Forum. Includes forums for common molecular biology techniques.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 11-15-2012, 01:55 PM
Pipette Filler
Points: 62, Level: 1 Points: 62, Level: 1 Points: 62, Level: 1
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Nov 2012
Posts: 2
Thanks: 2
Thanked 0 Times in 0 Posts
Default Problems in PCR "microsatellites" trubleshooting



Greetings .
Ive been trying to optimize the PCR condition\mix for a few SSR primers I will be using for a population genetics study in insects , The primers were used only in one paper ( which is the same one where they isolated the microsatellites ) .
So I spent sometime troubleshooting My PCRs and I ended up with some of them working well, and other are showing very clear bands , but out of the expected size ( some times its about 100 bp ) , is it logical to have a band which is that far from the expected size ??
the expected size was mentioned in one paper only and were tested in a population in the Netherlands ( and I'm using them in a population from Germany ) .
so is it logical to find such differences in using SSRs ?? or in case of optimizing pcr for microsatellites and I end up with a band which is out of range ..should I assume that I'm amplifying something rather than the targeted sequence ?

regards
Reply With Quote
Reply

Tags
entomology , microsatellites , pcr , pcr troubleshooting , population genetics , problems , ssr , trubleshooting


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Plasmid-stability? Fragment-deletion? I'm in dire need to identify and solve problems TGS Molecular Cloning Forum 3 10-22-2012 09:17 PM
Research News - Infants with persistent crying problems more likely to have behavior moleculardude Science News and Views 0 04-21-2011 11:14 AM
problems with growing healthy Arabidopsis plants Monika Kuzma Arabidopsis and Plant Biology 1 02-19-2007 03:55 PM
Problems with growing healthy Arabidopsis plants - light intesity? Karl Lundy Arabidopsis and Plant Biology 0 02-07-2007 05:11 PM


All times are GMT. The time now is 09:06 AM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.11110 seconds with 16 queries