| | |||||||
| Register | Search | Today's Posts | Mark Forums Read |
| Molecular Biology Techniques Molecular Biology Forum. Includes forums for common molecular biology techniques. |
| | LinkBack | Thread Tools | Display Modes |
|
#1
| |||||||||||
| |||||||||||
| Greetings . Ive been trying to optimize the PCR condition\mix for a few SSR primers I will be using for a population genetics study in insects , The primers were used only in one paper ( which is the same one where they isolated the microsatellites ) . So I spent sometime troubleshooting My PCRs and I ended up with some of them working well, and other are showing very clear bands , but out of the expected size ( some times its about 100 bp ) , is it logical to have a band which is that far from the expected size ?? the expected size was mentioned in one paper only and were tested in a population in the Netherlands ( and I'm using them in a population from Germany ) . so is it logical to find such differences in using SSRs ?? or in case of optimizing pcr for microsatellites and I end up with a band which is out of range ..should I assume that I'm amplifying something rather than the targeted sequence ? regards |
| Tags |
| entomology , microsatellites , pcr , pcr troubleshooting , population genetics , problems , ssr , trubleshooting |
| Thread Tools | |
| Display Modes | |
|
|
| | ||||
| Thread | Thread Starter | Forum | Replies | Last Post |
| Plasmid-stability? Fragment-deletion? I'm in dire need to identify and solve problems | TGS | Molecular Cloning Forum | 3 | 10-22-2012 09:17 PM |
| Research News - Infants with persistent crying problems more likely to have behavior | moleculardude | Science News and Views | 0 | 04-21-2011 11:14 AM |
| problems with growing healthy Arabidopsis plants | Monika Kuzma | Arabidopsis and Plant Biology | 1 | 02-19-2007 02:55 PM |
| Problems with growing healthy Arabidopsis plants - light intesity? | Karl Lundy | Arabidopsis and Plant Biology | 0 | 02-07-2007 04:11 PM |