AIDS, namely acquired immune deficiency syndrome, is a kind of infectious diseases caused by HIV infection, there is no therapeutic method at present.
In AIDS vaccine research and development, scientists around the world have explored strenuously for 20 years, so far only four AIDS vaccine has been proceeded to the third or the second phase of clinical trials,among which three are failed, another oneís overall protection ratio is only 31%
From Genomeweb news, according to Canadian public health researchers, using the second generation sequencing technology to sequence the AIDS virus gene in blood cake, detecting resistance of HIV virus at low cost. The cost of this method is lower than genotype of plasma samples based on PCR, also can understand resistance information of AIDS virus more comprehensively.
A preliminary research published in Antiviral Therapy, roche 454 GS FLX system was used in Canadian national AIDS virus and antiretroviral laboratory to sequence 48 patient's blood who was infected with HIV - 1 virus,for researching the virus resistance. These samples were taken from a study in 2007, these patients were diagnosed with HIV - 1 but not yet to accept any treatment.
The results showed that 454 sequencing results agree with Sanger method, even detected a mutation which Sanger method had not detected. The author said: "mixed sample of 454 pyrophosphoric acid sequencing is a very effective and economic second generation sequencing method, especially used in identification for ratio of drug resisitance of dried blood spotís HIV drug resistance. This method helps to get HIV drug resistance data with low cost and high flux and the limited resources and configuration."
The director of Canadian health bureau HIV genetics laboratory James Brooks said, "although the cost of 454 sequencing technology is relatively higher than the other two generations of sequencing system, but the length of the 454 is very suitable for HIV genome segment we focus on. And Ion Torrent technology also have certain specialty, but now it's reading is not long enough. So this time 454 became the winner." In addition to the cheaper price, compared with the traditional method, the second generation sequencing method can detect mutant gene more sensitively.
According to the statistics of world health organization (WHO), by the end of 2011, there were 34.2 million HIV carriers, about 1.7 million people died of AIDS in 2011, including 230000 children were blow 15 years old of. The consensus of scientific community is developing a vaccine against HIV is the most effective way. However, since human found HIV for the first time in 1983, AIDS vaccine research and development repeatedly failed.
Melinda gates foundationís statistics showed that about 190 AIDS vaccine experiment have been carried out around the world by the end of 2010. RV144 is currently the only AIDS vaccine proved to be with rate of protection of 31.2% . The comment within the industry indicated that the scientific value of this latest found is greater than its actual value.