Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Molecular Biology Techniques
Register Search Today's Posts Mark Forums Read

Molecular Biology Techniques Molecular Biology Forum. Includes forums for common molecular biology techniques.


Lamp

Lamp - Molecular Biology Techniques

Lamp - Molecular Biology Forum. Includes forums for common molecular biology techniques.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 11-06-2012, 10:22 AM
Pipette Filler
Points: 504, Level: 10 Points: 504, Level: 10 Points: 504, Level: 10
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Mar 2012
Posts: 2
Thanks: 0
Thanked 0 Times in 0 Posts
Default Lamp



hi,

i'm working with LAMP primers. all was well in the beginning but recently i have strated getting bands in negative control and the banding pattern is not clear ladder like as before. its hazzy and bands in the ladder are not clear? i have ruled out the contamination factor now. and sometimes surprisingly, i get band in one tube of negative control and no band in the other tube though they have the same reaction mix (without DNA).

Please advice me if anyone has a solution to this ?

Thanks:huh
Reply With Quote
  #2  
Old 03-07-2014, 05:01 PM
Pipette Filler
Points: 88, Level: 1 Points: 88, Level: 1 Points: 88, Level: 1
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Feb 2014
Posts: 5
Thanks: 0
Thanked 0 Times in 0 Posts
Default Re: Lamp

Quote:
Originally Posted by samom View Post
hi,

i'm working with LAMP primers. all was well in the beginning but recently i have strated getting bands in negative control and the banding pattern is not clear ladder like as before. its hazzy and bands in the ladder are not clear? i have ruled out the contamination factor now. and sometimes surprisingly, i get band in one tube of negative control and no band in the other tube though they have the same reaction mix (without DNA).

Please advice me if anyone has a solution to this ?

Thanks:huh
UV your working area and try again
Reply With Quote
Reply

Tags
lamp


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
(LAMP) Problems with Loop-mediated isothermal amplification Nickdn DNA Techniques 107 04-15-2014 03:26 AM
Specificity of LAMP primers Harsh Molecular Biology Techniques 3 05-30-2012 07:13 AM
restriction digests for HIV -1 LAMP products Edok Molecular Biology Techniques 1 03-30-2012 09:13 AM
jakie paliwo polskie do lamp naftowych/benzynowych cisnieniowych potek Forum Chemia 0 11-19-2005 01:45 PM
fluorescent lamp + spectral analyse jezzman81@hotmail.com Forum Physik 0 02-27-2005 02:00 PM


All times are GMT. The time now is 01:21 AM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.11814 seconds with 16 queries