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C-peptide ELIZA

C-peptide ELIZA - Molecular Biology Techniques

C-peptide ELIZA - Molecular Biology Forum. Includes forums for common molecular biology techniques.


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Old 10-26-2012, 09:41 AM
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Default C-peptide ELIZA



I am trying to develop a sandwich C-peptide ELIZA.
I have used a mouse monoclonal anti-C peptide antibody [1H8] as a capture antibody (Abcam - ab8297) - immunogen recominant full length protein.
The C-peptide protein (57-87) I'm using to generate a standard curve is also from abcam - ab93903.
The detection antibody is anti-C peptide (biotin labelled) - ab48303 - rabbit polyclonal - immunogen corresponding to internal sequence amino acids 57-87 of human c-peptide which is identical to the c-peptide protein I'm using.
However I can generate a standard curve - I'm only getting a very low uniform signal on analysis which I believe is due to a problem with the detection antibody.

Has anyone any thoughts or suggestions? Has anyone developed a c-peptide ELIZA and recommend a duoset of antibodies that work? Many thanks
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Old 10-29-2012, 04:35 PM
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Default Re: C-peptide ELIZA

In immunoenzymatic capture method, to know only the used antibodies is not enough for to evaluate an application method, but it's necessary know structure of the whole home kit.

Let me know
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