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Deletion of Dtr gene in a plasmid

Deletion of Dtr gene in a plasmid - Molecular Biology Techniques

Deletion of Dtr gene in a plasmid - Molecular Biology Forum. Includes forums for common molecular biology techniques.


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Old 10-04-2012, 02:15 AM
Pipette Filler
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Default Deletion of Dtr gene in a plasmid



Hi,

In a given donor you delete a Mpf (mating pair formation) tra gene and mate it with a recipient which has a mutation in it. It can't complement it, however when the donor plasmid lacks a Dtr Mpf it can complement the recipient, Why??

Anybody has an idea?

Is it possible that just by formation of the Pilus without Dtr tra genes something can be transferred ?

Thanks in advance
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