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Conventional PCR

Conventional PCR - Molecular Biology Techniques

Conventional PCR - Molecular Biology Forum. Includes forums for common molecular biology techniques.


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Old 10-01-2012, 06:08 PM
Pipette Filler
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Default Conventional PCR



Dear Sir:
Please can you help me to tell how can I convert the protocol of real time PCR to conventional PCR. I have primers for mouse Ogg1, Many trails I did but I failed to obtain a result by conventional PCR. If we need any information about my primers I will provide them.
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Old 10-22-2012, 09:40 PM
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Default Re: Conventional PCR

Real time PCR and "conventional" PCR are the same the only difference is how the reaction is measured. What exactly do you mean by convert? o.O
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Old 10-23-2012, 02:40 PM
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Default Re: Conventional PCR

I meant the temperature and time for denaturation, annealing, and extension stages. I fail to obtain result by using the same temperature and time mentioned by the article.
in the article they used Real time PCR while I use conventional.
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