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Equine Neutrophil Isolation

Equine Neutrophil Isolation - Molecular Biology Techniques

Equine Neutrophil Isolation - Molecular Biology Forum. Includes forums for common molecular biology techniques.


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Old 09-19-2012, 11:36 PM
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Default Equine Neutrophil Isolation



Hello,

I have been isolating neutrophils from human blood and my protocol works well. However, I have been finding the isolation much more difficult in equine blood. I am having trouble with the neutrophils clumping. I've searched the literature to find out what might be causing this, but I haven't found the answer. Does anyone have ideas?
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Old 09-20-2012, 07:13 AM
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Default Re: Equine Neutrophil Isolation

Neutrophils clumping can be observed in blood anticoagulated with citrate or heparin. Centrifuged sample at low speed and plasma replacement with PBS or saline at 37 C, and as an alternative, holding blood specimen at 37 C that prevented agglutination. This phenomenon can be linked presence of IgM on neutrophils surfaces.
A method for optimal and not contaminated neutrophils separation from the peripheral blood, from me experimented, you will find in:
[Only registered users see links. ]
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