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| Hi, i cut puc 18 with Ecor I and Hind III separatly (incubate just 30 min in dry bath) and run on agarose gel (2%) at 75v for 2.30hrs. but there were not a big diatance different with uncut plasmid. it this result correct. i have a dout a boubt it. plz clarify this . |
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