I am planning to do some bisulphite sequencing PCR. I have seen in several protocols a recommendation to use M13 primer tails in order to simplify the sequencing aspect of the protocol. I do not want to do cloning (although I know this will introduce the M13 via the vector) but prefer a direct sequencing approach.
My questions are: do I simply append the M13 sequences to the 5' end of my primer sequences? Or do I need to modify one or both sets of sequences for compatibility?
Next: how will this affect my cycling conditions? Which M13 sequences to use (I found several variants online)?
Many thanks for your help - this is hurting my head!