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M13 sequencing primers bisulphite PCR

M13 sequencing primers bisulphite PCR - Molecular Biology Techniques

M13 sequencing primers bisulphite PCR - Molecular Biology Forum. Includes forums for common molecular biology techniques.


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Old 08-16-2012, 10:49 AM
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Default M13 sequencing primers bisulphite PCR



Hello,

I am planning to do some bisulphite sequencing PCR. I have seen in several protocols a recommendation to use M13 primer tails in order to simplify the sequencing aspect of the protocol. I do not want to do cloning (although I know this will introduce the M13 via the vector) but prefer a direct sequencing approach.

My questions are: do I simply append the M13 sequences to the 5' end of my primer sequences? Or do I need to modify one or both sets of sequences for compatibility?

Next: how will this affect my cycling conditions? Which M13 sequences to use (I found several variants online)?

Many thanks for your help - this is hurting my head!

Emma
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bisulphite , m13 , pcr , primers , sequencing


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