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Positive result in negative control

Positive result in negative control - Molecular Biology Techniques

Positive result in negative control - Molecular Biology Forum. Includes forums for common molecular biology techniques.


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Old 08-08-2012, 04:19 AM
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Default Positive result in negative control



Hi all,

Hope you guys doing fine.. I am having a problem in running LAMP amplification reaction. I designed a LAMP primer set (F3, B3, FIP, BIP, LF and LB) and decided to go for a negative control run first and then the positive run. The results were perfect as expected.

Primers were freeze thawed. When I tried running the reaction with the primers, negative control run yielded positive result.

Did I make any mistake? Is it like, due to freeze thawing, primers have formed some secondary structures???? How can I get those primers worked again??

Please help me//

Cheers !!
Harsh
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