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Neuritogenesis Loading controls
I am looking at neurite outgrowth for PC12 and SHSY5Y cells.
I have 2 questions:
1. I am interested in the phosphorylation status of certain genes such as Erk and Creb. Can someone clarify if using the non-phosphorylated proteins as loading controls is sufficient, even at later time points after treatment (24h-72h)?
2. If I want to measure expression of neuronal markers such as beta3 tubulin and GAP43, what loading controls are good? Are there any publications supporting this?
|controls , loading , neuritogenesis|
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