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gel extraction - plasmid

gel extraction - plasmid - Molecular Biology Techniques

gel extraction - plasmid - Molecular Biology Forum. Includes forums for common molecular biology techniques.


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Old 06-20-2012, 08:21 PM
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Default gel extraction - plasmid



Hi guys,
I'm isolating large plasmids from the environmetntal strain. However I've got contamination from chromosoem what I can observe as a smear on the agarose gel. I'm planing to cut it out from the gel and purify with gel extraction columns and later cut it randomly with restriction enzymes and clone it into vector. My worry is that fragment from chromosome might also contaminate this fragment during purification. What do you think???
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