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EMSA gel loading issue

EMSA gel loading issue - Molecular Biology Techniques

EMSA gel loading issue - Molecular Biology Forum. Includes forums for common molecular biology techniques.


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Old 06-14-2012, 06:21 AM
Pipette Filler
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Default EMSA gel loading issue



Lately I have been facing a strange problem while loading samples into the wells. My wells are well formed, I am flushing them properly and also doing a pre-run. My problem is that the sample is not setting down at the bottom of the well when I am loading and just hanging inside the well. There is no air bubble issue, I suppose, as samples are not going out (floating) of the well while loading.

I added extra glycerol to make it heavy, but nothing seems to be working.

What's wrong?
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