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EMSA gel loading issue
Lately I have been facing a strange problem while loading samples into the wells. My wells are well formed, I am flushing them properly and also doing a pre-run. My problem is that the sample is not setting down at the bottom of the well when I am loading and just hanging inside the well. There is no air bubble issue, I suppose, as samples are not going out (floating) of the well while loading.
I added extra glycerol to make it heavy, but nothing seems to be working.
|emsa , gel , issue , loading|
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