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#1
06-14-2012, 06:21 AM
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 EMSA gel loading issue Lately I have been facing a strange problem while loading samples into the wells. My wells are well formed, I am flushing them properly and also doing a pre-run. My problem is that the sample is not setting down at the bottom of the well when I am loading and just hanging inside the well. There is no air bubble issue, I suppose, as samples are not going out (floating) of the well while loading. I added extra glycerol to make it heavy, but nothing seems to be working. What's wrong?  |
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| emsa , gel , issue , loading |
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