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LightCycler real time - Negative fluorescence

LightCycler real time - Negative fluorescence - Molecular Biology Techniques

LightCycler real time - Negative fluorescence - Molecular Biology Forum. Includes forums for common molecular biology techniques.


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Old 05-24-2012, 10:52 AM
Pipette Filler
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Default LightCycler real time - Negative fluorescence



Hi,

Has anyone seen a dip in fluorescence prior to the amplification curve running real time PCR on the lightcycler?

The fluorescence tends to increase slightly at the beginning of the run and then slowly decreases, sloping to below 0 before the amplication curve. Is there anyway to solve this? is it a software thing?

I've attached an example pic so you can see what I mean.

Thanks in advance
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File Type: gif Sloping baseline.gif (17.6 KB, 0 views)
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