I am a new user of real time PCR an I really need some help to choose the best method to analyze my data.
In my experiments I´m injecting an exogenous gene (GFP) in some animals (using a particular vector), and now I want to quantify the relative expression of this transgene in some tissues.
Initially, I´m thinking to aplly the 2^-ddCt method but I have a problem... What should I use as a calibrator? The nontreated animals do not have transgene expression, so it´s not correct to use it as calibrator... Am I right??
What method should I use to analyse my data?
If anyone can help me, please let me know.
Thanks in advance,