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regarding PCR product

regarding PCR product - Molecular Biology Techniques

regarding PCR product - Molecular Biology Forum. Includes forums for common molecular biology techniques.


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Old 04-08-2012, 02:07 PM
Pipette Filler
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Default regarding PCR product



Hi , im trying to clone a gene but the DNA template isolated was not good. after the 1st PCR i was getting a faint band on the size expected. so i eluted that PCR product and did a second PCR using the amplified gene. now im getting good amplification however there is a slight smear near the product band. can i use this product for transformation by selectively cutting the gel and eluting my desired product? are there chances of non specific product ligating into the vector?
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